Ultrastructural Remodeling of the Neurovascular Unit in the Female Diabetic db/db Model—Part III: Oligodendrocyte and Myelin

Neuroglia

Hayden, Melvin R.¹  Grant, Deana G.²  Aroor, Aranyra³  DeMarco, Vincent G.⁴ 

[1] Diabetes and Cardiovascular Center, University of Missouri School of Medicine, Columbia, MO 65212, USA;Division of Endocrinology and Metabolism, Department of Medicine, University of Missouri, Columbia, MO 63212, USA;Electron Microscopy Core Facility, University of Missouri, Columbia, MO 65211, USA;Research Service, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65212, USA

关键词: db/db mouse model;    myelin;    neurovascular unit;    oligodendrocyte;    subcortical white matter;    type 2 diabetes;   

DOI  :  10.3390/neuroglia1020024

学科分类：医学解剖学

来源: mdpi

【 摘 要 】

Obesity, insulin resistance, and type 2 diabetes mellitus are associated with diabetic cognopathy. In this study, we tested the hypothesis that neurovascular unit(s) (NVU), oligodendrocytes, and myelin within cerebral cortical grey matter and deeper transitional zone regions between the cortical grey matter and white matter may be abnormal. The monogenic (Leprdb) female diabetic db/db [BKS.CgDock7m +/+ Leprdb/J] (DBC) mouse model was utilized for this ultrastructural study. Upon sacrifice (20 weeks of age), left-brain hemispheres of the DBC and age-matched non-diabetic wild type control C57BL/KsJ (CKC) mice were immediately immersion-fixed. We found prominent remodeling of oligodendrocytes with increased nuclear chromatin condensation and volume and increased numbers of active myelination sites of the cytoplasm in transition zones. Marked dysmyelination with outer myelin lamellae sheath splitting, separation, and ballooning with aberrant mitochondria in grey matter and similar myelin remodeling changes with marked disarray with additional axonal collapse in transitional zones in DBC as compared to CKC models.

【 授权许可】

CC BY