Proceedings | |
Microchannel Fabrication Using A Photo-Patternable Adhesive Material for Recording Conformational Changes of KcsA Channel with the Diffracted X-ray Tracking Method | |
Tabuchi, Tomoki1  Hirai, Yoshikazu2  Shimizu, Hirofumi3  Mori, Yasuaki4  | |
[1] Author to whom correspondence should be addressed.;Department of Micro Engineering, Kyoto University, Kyoto 605-8540, Japan;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9â12 September 2018;School of Medical Sciences, University of Fukui, Fukui 910-1193, Japan | |
关键词: microfluidics; diffracted X-ray tracking method; KcsA ion channel; adhesive bonding; | |
DOI : 10.3390/proceedings2130972 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: mdpi | |
【 摘 要 】
Diffracted X-ray tracking (DXT) method can trace conformational changes of KcsA potassium ion channel during gating by recording position of diffraction spot from a gold nanocrystal attached to the channel as a movie. For high-resolution imaging under controlled microenvironments for KcsA channels, we report a microfluidic device consisting of two SiN membrane windows bonded with a photo patternable adhesive material. The reduced signal-to-background ratio as well as suitable adhesive material thickness for the microchannel are discussed in the experiment at the synchrotron radiation facility.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO201910252797376ZK.pdf | 750KB | download |