Microchannel Fabrication Using A Photo-Patternable Adhesive Material for Recording Conformational Changes of KcsA Channel with the Diffracted X-ray Tracking Method

Proceedings

Tabuchi, Tomoki¹  Hirai, Yoshikazu²  Shimizu, Hirofumi³  Mori, Yasuaki⁴ 

[1] Author to whom correspondence should be addressed.;Department of Micro Engineering, Kyoto University, Kyoto 605-8540, Japan;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018;School of Medical Sciences, University of Fukui, Fukui 910-1193, Japan

关键词: microfluidics;    diffracted X-ray tracking method;    KcsA ion channel;    adhesive bonding;   

DOI  :  10.3390/proceedings2130972

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Diffracted X-ray tracking (DXT) method can trace conformational changes of KcsA potassium ion channel during gating by recording position of diffraction spot from a gold nanocrystal attached to the channel as a movie. For high-resolution imaging under controlled microenvironments for KcsA channels, we report a microfluidic device consisting of two SiN membrane windows bonded with a photo patternable adhesive material. The reduced signal-to-background ratio as well as suitable adhesive material thickness for the microchannel are discussed in the experiment at the synchrotron radiation facility.

【 授权许可】

CC BY