Improved biphasic calcium phosphate combined with periodontal ligament stem cells may serve as a promising method for periodontal regeneration

American Journal of Translational Research

Han Shi¹ 

[1] Department of Orthodontics, School Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

关键词: Periodontal ligament;    stem cells;    biphasic calcium phosphate;    scaffold materials;    tissue engineering;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

The study aimed to investigate if a construct of porous improved biphasic calcium phosphate (BCP) combined with osteogenically induced periodontal ligament stem cells (PDLSCs) could repair periodontal defect. Human PDLSCs were cultured, identified, and osteogenically induced, and then seeded on the improved BCP, assessed by scanning electron microscopy and MTT method. Afterwards, PDLSC-seeded scaffolds were used in six Beagle dogs’ alveolar bone dehiscence model, and new bone formation was assessed by three-dimensional micro-computed tomographic imaging, fluorescence, and light microscopy at 4th, 8th and 12th week postoperatively. Results showed that PDLSCs were positive for STRO-1 and vimention, while negative for cytokeratin. They had the capacity to undergo osteogenic differentiation in vitro. When seeded on the improved BCP, PDLSCs exhibited significantly great viability. The use of PDLSC-seeded improved BCP promoted effective periodontal regeneration. In conclusion, this study demonstrates the potential of improved BCP combined with PDLSCs as a promising method for periodontal regeneration.

【 授权许可】

CC BY-NC