Molecular characteristics of Staphylococcus aureus isolates from food surveillance in southwest China

BMC Microbiology

Zushun Yang¹  Yidan Guo²  Zhishuo Mo³  Feng Liao⁴  Lu Fan⁵  Wenpeng Gu⁶  Xiaoqing Fu⁷ 

[1] Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, The Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree Shrew, Kunming, China;Centre for Sanitary Inspection, Yunnan Provincial Centre for Disease Control and Prevention, Kunming, China;Department of Acute Infectious Diseases Control and Prevention, Yunnan Provincial Centre for Disease Control and Prevention, Kunming, China;Department of Respiratory Medicine, The First Peoples Hospital of Yunnan province, Kunming, China;Institute of Medical Biology, The Chinese Academy of Medical Science and Peking Union Medical College & Department of Acute Infectious Diseases Control and Prevention, Yunnan Provincial Centre for Disease Control and Prevention, Kunming, China;The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China;The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China

关键词: Staphylococcus aureus;    Food surveillance;    Southwest China;   

DOI  :  10.1186/s12866-018-1239-z

学科分类：微生物学和免疫学

来源: BioMed Central

【 摘 要 】

Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases in the world. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and spa typing methods were used to characterize Staphylococcus aureus isolates from food surveillance during 2013–2015 in southwest China, and Staphylococcal cassette chromosome mec (SCCmec) typing was used for methicillin-resistant S. aureus (MRSA). Isolates were also examined for their antibiotic resistance and carriage of virulence genes. Isolation rate of S. aureus was 2.60% during the three years’ surveillance and 29.50% of them were MRSA. All the S. aureus had hla genes (100%), 14.34% of the strains had tst, and 16.73% had PVL. 163 PFGE-SmaI patterns, 41 ST types and 36 spa types were obtained for all the S. aureus. Among them, ST6-t701 (13.15%), ST7-t091 (12.75%), ST59-t437 (9.96%) and ST5-t002 (7.57%) were the prevalent genotypes. Most of MRSA in this study belonged to SCCmec IV and V, accounted for 74.32% and 20.27% respectively. ST6-SCCmec IV-t701 (36.50%) was the most prevalent clone among isolates from food, followed by ST59-SCCmec V-t437 (20.30%), ST5-SCCmec IV-t002 (12.20%) and ST59-SCCmec IV-t437 (12.20%). Some strains had the identical PFGE patterns, ST and spa types with isolates from patients. S. aureus isolated from food in southwest China displayed heterogeneity. Isolates had the same genotype profiles with isolates from patients, indicating high homology.

【 授权许可】

CC BY