PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection

Jundishapur Journal of Microbiology

Carolina Resendiz-Nava¹  Yajaira Esquivel-Hernandez² 

[1] Department of Research and Graduate Studies in Food, Autonomous University of Queretaro, Santiago de Querétaro, Mexico;National Autonomous University of Mexico, Mexico City, Mexico

关键词: PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection;    Salmonella;    invA;    PCR;    Detection;    Citrobacter;    16S rRNA;    ttrA;    ttrC;   

DOI  :  10.5812/jjm.68764

学科分类：微生物学和免疫学

来源: Jundishapur Journal of Microbiology

【 摘 要 】

Salmonella surveillance relies on invA polymerase chain reaction (PCR) assays for the rapid detection of Salmonella; however, false-positive results have been reported using this method.To evaluate the performance and specificity of the published and validated PCR protocols targeting invA gene for the detection of Salmonella.The performance and specificity of 11 different PCR primer sets were evaluated using Salmonella type strains and Citrobacter spp., Escherichia coli and Serratia spp. isolates recovered during a Salmonella surveillance program.It was revealed that the published PCR protocols using validated primers targeting invA and 16S rRNA genes generated false-positive signals. Importantly, a protocol targeting the ttrA/C genes was able to discriminate Salmonella and non-Salmonella isolates.Detection of Salmonella spp. by means of invA PCR amplification is not reliable. In fact, false-positive results are commonly obtained from Citrobacter, E. coli and Serratia isolates. It is recommended to use other loci, such as ttrA/C genes, for the accurate and reliable detection of Salmonella.

【 授权许可】

CC BY-NC