Comparative secretome analysis of human follicular dermal papilla cells and fibroblasts using shotgun proteomics

BMB Reports

Dong Hun Lee^2,3¹  Chong Hyun Won^1²  Oh Sang Kwon^2,3³  Hyeon Gyeong Yoo^2,3⁴  Jin Ho Chung^2,3⁵  Kyu Han Kim^2,3⁶  Yong Jung Kang^2,3⁷ 

[1] D Center, Gyeonggi-do 446-729^5;Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736^1;Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital^2;Dermatologic Drug Research, Pharmaceutical Research Institute, Amore Pacific R&^4;Division of Life Science, Korea Basic Science Institute, Daejeon 305-333^6;Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Korea^7;Institute of Dermatological Science, Seoul National University College of Medicine, Seoul 110-744^3

关键词: Dermal fibroblast;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

The dermal papilla cells (DPCs) of hair follicles are known to secrete paracrine factors for follicular cells. Shotgun proteomic analysis was performed to compare the expression profiles of the secretomes of human DPCs and dermal fibroblasts (DFs). In this study, the proteins secreted by DPCs and matched DFs were analyzed by 1DE/LTQ FTICR MS/MS, semi-quantitatively determined using emPAI mole percent values and then characterized using protein interaction network analysis. Among the 1,271 and 1,188 proteins identified in DFs and DPCs, respectively, 1,529 were further analyzed using the Ingenuity Pathway Analysis tool. We identified 28 DPC-specific extracellular matrix proteins including transporters (ECM1, A2M), enzymes (LOX, PON2), and peptidases (C3, C1R). The biochemically- validated DPC-specific proteins included thrombospondin 1 (THBS1), an insulin-like growth factor binding protein3 (IGFBP3), and, of particular interest, an integrin beta1 subunit (ITGB1) as a key network core protein. Using the shotgun proteomic technique and network analysis, we selected ITGB1, IGFBP3, and THBS1 as being possible hair-growth modulating protein biomarkers.

【 授权许可】

Unknown