Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica

Revista de microbiologia

Cotârle, Mihaela¹  Negoi, Teodor Gh²  Bahrim, Gabriela E³ 

[1] Romanian Polar Research Institute, Bucharest, Romania;University Dunrea de Jos, Galai, Romania;University of Copenhagen, Denmark

关键词: Streptomyces sp;    East Antarctica;    cold-adapted strain;    cold active proteases and amylase.;   

DOI  :  10.1590/S1517-83822011000300005

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.

【 授权许可】

CC BY-NC