A Reliable and Sensitive Method Using Cyclic Voltammetry for the Detection of Airborne Fungi

Proceedings

Cserna, Dominique¹  Ettenauer, Jörg²  Zuser, Karen³  Pfeiffer, Sandra⁴  Brandl, Martin⁵ 

[1] Author to whom correspondence should be addressed.;Department for Integrated Sensor Systems, Center for Water and Environmental Sensors, Danube University Krems, 3500 Krems, Austria;Division Microbiological Diagnostics for Water and Health, Physiology und Microbiological Diagnostics, Karl Landsteiner University of Health Sciences, 3500 Krems, Austria;IMC FH Krems, University of Applied Sciences, 3500 Krems, Austria;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.

关键词: airborne fungi;    Aspergillus niger;    biosensor;    cellulolytic activity;    cyclic voltammetry;    electrochemical detection;   

DOI  :  10.3390/proceedings2130959

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Fungi are widespread throughout the environment and can cause health as well as indoor problems. One of the most common fungi involved in building damage are Aspergillus sp. This study describes a rapid method for the detection of fungal spores. It is based on the ability of fungi to produce the enzyme cellulase, which can cleave the substrate Aminophenyl-β-d-cellobioside (APC) resulting into 4-Aminophenyl (AP). This cleavage product can then be oxidized leading to an increased output current signal. In the experimental setup several growth media and different parameters were tested over time, for instance growth conditions like pH or incubation temperature. Furthermore, various spore concentrations, which are related to the cellulase activities, were examined. This method presents a technique that makes it possible to specifically detect low concentrations of fungi spores in short time.

【 授权许可】

CC BY