MiR-378a-5p acts as a tumor suppressor in renal cell carcinoma and is associated with the good prognosis of patients

American Journal of Translational Research

Kaihao Liu¹  Xiang Pan²  Liwen Zhao³  Jing Quan⁴ 

[1] Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, P. R. China;Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Clinical College of Anhui Medical University, Shenzhen 518036, Guangdong, P. R. China;Guangzhou Medical University, Guangzhou 511436, Guangdong, P. R. China;Shantou University Medical College, Shantou 515041, Guangdong, P. R. China

关键词: MicroRNA;    miR-378a-5p;    renal cell carcinoma;    oncogene;    prognosis biomarker;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Renal cell carcinoma (RCC) is a common cancer that accounts for about 1.6% of all malignancies. Accumulating evidence has shown that miRNAs may play important roles in the development of cancers and that these same miRNAs may serve as diagnostic and prognostic biomarkers. The role of the miRNA miR-378a-5p in RCC, however, has been largely unexplored. In our study, we have demonstrated that miR-378a-5p expression was decreased in renal tissues and in RCC cell lines compared with corresponding expression levels in normal renal tissues and in the 293-T cell line. Functional studies in two RCC cell lines (ACHN and 786-O) have indicated that miR-378a-5p overexpression attenuated cell proliferation, migration, and invasion while promoting cell apoptosis. Inhibition of miR-378a-5p expression, on the other hand, promoted cell proliferation, migration, and invasion while reducing cell apoptosis. Additionally, in 42 cases of renal cancer formalin-fixed paraffin-embedded specimens, patients with higher expression levels of miR-378a-5p had significantly longer overall survival rates (P<0.05) than patients with lower miR-378a-5p expression levels. Thus, in this study, we have shown that miR-378a-5p can serve as a tumor suppressor and a potential prognostic biomarker in RCC.

【 授权许可】

CC BY-NC