Identification of miR-18a-5p as an oncogene and prognostic biomarker in RCC

American Journal of Translational Research

Jing Quan¹  Zuwei Li²  Liang Zhou³  Xiang Pan⁴  Yulin Lai⁵ 

[1] Department of Urology, Anhui Medical University, Hefei 230032, Anhui, China;Department of Urology, Guangzhou Medical University, Guangzhou 511436, Guangdong, China;Department of Urology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China;Department of Urology, Shantou University Medical College, Shantou 515041, Guangdong, China;The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong, China

关键词: MicroRNAs;    miR-18a-5p;    renal cell carcinoma;    oncogene;    prognosis biomarker;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Background: RCC is a malignant tumor that originates from renal tubular epithelial cells, accounting for nearly 90% of renal malignancies and 3% of adult malignancies. It was reported that more than 30-40% of patients with early localized RCC still have recurrence and metastasis after receiving radical surgery. miRNAs are an endogenous non-coding small RNAs that play an important role in the regulation of tumor cell proliferation, differentiation and apoptosis. Methods: In our study, RT-qPCR, CCK-8 assay, wound scratch assay, transwell assay and flow cytometry assay were designed to identify the expression and functions of miR-18a-5p in RCC. Moreover, we collected the survival data from The Cancer Genome Atlas to predict and clarify the prognostic functions of miR-18a-5p in RCC. The correlation between miR-18a-5p expression and clinicopathological variables or overall survival was analyzed by 42 formalin-fixed paraffin-embedded (FFPE) renal cancer samples. Results: The expression of miR-18a-5p in RCC tissues and cell lines was elevated. Further researches suggested that upregulation of miR-18a-5p had a positive effect on RCC cell proliferation, migration, invasion and inhibition of apoptosis, while down-regulation of miR-18a-5p neutralized the effect. In addition, Data of TCGA and prognostic analysis of FFPE RCC samples revealed that high miR-18a-5p expression patients had significantly poorer survival. Conclusions: These results demonstrated that miR-18a-5p functioned as an oncogene and prognostic biomarker in RCC.

【 授权许可】

CC BY-NC