Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan

Revista de microbiologia

Khattak, Yasir¹  Khan, Muhammad Tahir²  Khan, Anwar Sheed³  Ali, Sajid⁵  Sajjad, Wasim⁶  Irfan, Muhammad⁷  Abduljabbar,⁸  Ahmed, Sajjad⁹ 

[1] Technology, Islamabad, Pakistan;Capital University of Science &Khyber Medical University Peshawar, Peshawar, Pakistan;National University of Medical Sciences, Rawalpindi, Pakistan;Provincial TB Reference Laboratory, Peshawar, Pakistan;Sarhad University of Science and Information Technology, Peshawar, Pakistan;University of Haripur, Haripur, Pakistan;University of Peshawar, Peshawar, Pakistan;amp

关键词: GeneXpert;    LED-FM microscopy;    Sensitivity;    Specificity.;   

DOI  :  10.1016/j.bjm.2018.02.011

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.

【 授权许可】

CC BY-NC