Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests

Revista de microbiologia

Cargnelutti, Juliana Felipetto¹  Brum, Mário Celso Sperotto² 

[1] Universidade Federal de Santa Maria, Santa Maria, Brasil;Universidade Federal do Pampa, Uruguaiana, Brasil

关键词: West Nile virus;    WNV;    M/E glycoproteins;    stable expression;    diagnostic.;   

DOI  :  10.1590/S1517-83822011000300040

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.

【 授权许可】

CC BY-NC