Endothelial Dysfunction in Breast Cancer In-Vivo Model

Proceedings

Aktaş, Safiye¹  Çakır, Yasemin²  Çalışır, Meryem³  Barış, Mustafa⁴  Tütüncü, Merve⁵  Elkar, Buğra⁶ 

[1] Author to whom correspondence should be addressed.;Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir 35340, Turkey;Department of Laboratory Animals, Institute of Health Sciences, Dokuz Eylul University, Izmir 35340, Turkey;Department of Radiology, School of Medicine, Dokuz Eylul University, Izmir 35340, Turkey;Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018;School of Medicine, Dokuz Eylul University, Izmir 35340, Turkey

关键词: endothelial dysfunction;    breast cancer;    USG;    L-NAME;    cancer development;   

DOI  :  10.3390/proceedings2251536

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Although the endothelial dysfunction is related with tumor development, there is no consensus on the suppressive or supportive effect on tumor growth. The goal of the present study was to evaluate endothelial dysfunction related factors in animal breast cancer model that was developed by administrating endothelial nitric oxide synthase blocking agent, Nitro-L-arginine methyl ester hydrochloride (L-NAME). Endothelial dysfunction related main factors such as nitric oxide synthase, interleukin-6, vascular endothelial growth factor receptor-2 and vascular endothelial cadherin were investigated by immunohistochemically in tumor and carotid artery tissues. In tumor tissues apoptosis was determined by TUNEL assay. The level of endothelin-1 in blood was measured by ELISA. Ä°ntima-media thickness of carotid artery was evaluated with Doppler-USG measurements. As a result, in this study it was shown that vascular endothelial growth factor receptor-2, endothelin-1, endothelial nitric oxide synthase, interleukin-6, vascular endothelial cadherin and E-selectin molecules play a role in reducing breast tumor growth based on endothelial dysfunction.

【 授权许可】

CC BY