The Development of a Novel Diagnostic Assay That Utilizes a Pseudotyped Vesicular Stomatitis Virus for the Detection of Neutralizing Activity against Crimean-Congo Hemorrhagic Fever Virus

Japanese journal of infectious diseases

Yuto Suda¹  Stuart D. Dowall²  John Chamberlain³ 

[1] Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo;National Infection Service, Public Health England;Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases

关键词: biosafety level;    Crimean-Congo hemorrhagic fever virus;    pseudotyped virus;   

DOI  :  10.7883/yoken.JJID.2017.354

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Crimean-Congo hemorrhagic fever virus is a risk group 4 pathogen, which mandates the use of maximum containment facilities, often termed biosafety level 4 or containment level 4 when working with infectious materials. Diagnostic and research work involving live viruses in such laboratories is time-consuming and inconvenient, resulting in delays. Herein, we show that serum neutralizing activity against the virus can be measured in low-containment laboratories using a pseudotyped virus.

【 授权许可】

Unknown