Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

Revista de microbiologia

Saxena, Rajshree¹ 

[1] Amity University, Noida, India

关键词: Solid State Fermentation;    Agro-industrial wastes;    Amylase;    Enzyme optimization;    Stability;    Bacillus  ;    sp.;   

DOI  :  10.1590/S1517-83822011000400014

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.

【 授权许可】

CC BY-NC