BMC Microbiology | |
Gold nanoparticles attenuates bacterial sepsis in cecal ligation and puncture mouse model through the induction of M2 macrophage polarization | |
Thunnicha Ondee1  Saowapha Surawut2  Amornpun Sereemaspun3  Naunpun Sangphech4  Sujittra Taratummarat5  Tanapat Palaga6  Chau Tran Bao Vu7  | |
[1] Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;Department of Microbiology and Immunology and Research Unit of Oral Microbiology, Chulalongkorn University, Bangkok, Thailand;Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;Medical Microbiology, Interdisciplinary Program, Graduate School, Chulalongkorn University, Bangkok, Thailand;Medical Sciences Program, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;Oral Biology program, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand | |
关键词: Sepsis; Gold nanoparticles; Cecal ligation and puncture; Macrophage polarization; | |
DOI : 10.1186/s12866-018-1227-3 | |
学科分类:微生物学和免疫学 | |
来源: BioMed Central | |
【 摘 要 】
Gold nanoparticles (AuNP) have several biochemical advantageous properties especially for a candidate of drug carrier. However, the non-conjugated AuNP has a higher rate of cellular uptake than the conjugated ones. Spherical AuNP in a proper size (20–30 nm) is non-toxic to mice and shows anti-inflammatory properties. We tested if the administration of AuNP, as an adjuvant to antibiotics, could attenuate bacterial sepsis in cecal ligation and puncture (CLP) mouse model with antibiotic (imipenem/cilastatin). Indeed, AuNP administration at the time of CLP improved the survival, blood bacterial burdens, kidney function, liver injury and inflammatory cytokines (TNF-α, IL-6, IL-1β and IL-10). AuNP also decreased M1 macrophages (CD86 + ve in F4/80 + ve cells) and increased M2 macrophages (CD206 + ve in F4/80 + ve cells) in the spleens of sepsis mice. The weak antibiotic effect of AuNP was demonstrated as the reduction of E. coli colony after 4 h incubation. In addition, AuNP altered cytokine production of bone-marrow-derived macrophages including reduced TNF-α, IL-6 and IL-1β but increased IL-10 at 6 and 24 h. Moreover, AuNP induced macrophage polarization toward anti-inflammatory responses (M2) as presented by increased Arg1 (Arginase 1) and PPARγ with decreased Nos2 (inducible nitric oxide synthase, iNos) and Nur77 at 3 h after incubation in vitro. The adjuvant therapy of AuNP, with a proper antibiotic, attenuated CLP-induced bacterial sepsis in mice, at least in part, through the antibiotic effect and the induction of macrophage function toward the anti-inflammatory responses.
【 授权许可】
CC BY
【 预 览 】
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