Bafilomycin Prevented Curcumin-Induced Endoplasmic Reticulum (ER) Stress and Autophagy in MCF-7 Growth Hormone Positive (GH+) Breast Cancer Cells

Proceedings

Coker-Gurkan, Ajda¹  Karataş, Merve²  Arisan, Elif Damla³ 

[1] Author to whom correspondence should be addressed.;Department of Molecular Biology and Genetics, Science and Letters Faculty, Istanbul Kultur University, Ä°stanbul 34158, Turkey;Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018

关键词: curcumin;    growth hormone;    autophagy;    endoplasmic reticulum stress;    bafilomycin;   

DOI  :  10.3390/proceedings2251568

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Autocrine growth hormone (GH) induced cell proliferation, invasion-metastasis and drug resistance in breast cancer cells. Curcumin has an apoptotic effect on colon, melanoma, cervix, and breast cancer cells. Autophagy and endoplasmic reticulum (ER) stress are essential cellular processes activated under nutrient deprivation, pathogen infection and drug exposure. Our aim in this study is to investigate the time-dependent effect of curcumin on ER stress and autophagy and potential increase of curcumin efficiency by bafilomycin treatment. Autocrine GH expression triggered resistant profile against curcumin-induced cell viability loss in MCF-7 cells. However, this effect was prevented by the time-dependent manner in MCF-7 cells. In GH+ breast cancer cells bafilomycin increase curcumin-induced cell viability loss by MTT cell viability assay. In conclusion, autocrine GH-triggered curcumin resistance was overcome by autophagy inhibition condition by bafilomycin treatment in a dose-dependent manner in MCF-7 GH+ breast cancer cells.

【 授权许可】

CC BY