Significant therapeutic effects of adult human multipotent neural cells on spinal cord injury - ScienceDirect

Stem Cell Research

Hyun Nam^2,4,5,1¹  Kee-Hang Lee^1,2,5²  Jeong-Seob Won^1,2,5³  Kyung-A Lee^3⁴  Ji-Yoon Hwang^2,4,5⁵  Hee-Jang Pyeon^2,3,5,1⁶ 

[1] Department of Anatomy & Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea^3;Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, South Korea^1;Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, South Korea^4;Samsung Biomedical Research Institute, Samsung Medical Center, Seoul 06351, South Korea^6;Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, 16419, South Korea^5;Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, South Korea^2

关键词: Spinal cord injuries;    Adult stem cells;    Neural stem cells;    Microvasculature;    Chemokine1;   

DOI  :  10.1016/j.scr.2018.07.006

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Neural stem cells are emerging as a regenerative therapy for spinal cord injury (SCI), since they differentiate into functional neural cells and secrete beneficial paracrine factors into the damaged microenvironment. Previously, we successfully isolated and cultured adult human multipotent neural cells (ahMNCs) from the temporal lobes of epileptic patients. In this study, we investigated the therapeutic efficacy and treatment mechanism of ahMNCs for SCI using rodent models. When 1 × 106 ahMNCs were transplanted into injured spinal cords at 7 days after contusion, the injection group showed significantly better functional recovery than the control group (media injection after contusion), which was determined by the Basso, Beattie and Bresnahan (BBB) score. Although transplanted ahMNCs disappeared continuously, remained cells expressed differentiated neural cell markers (Tuj1) or astrocyte marker (GFAP) in the injured spinal cords. Moreover, the number of CD31-positive microvessels significantly increased in the injection group than that of the control group. The paracrine pro-angiogenic activities of ahMNCs were confirmed by in vitro tube formation assay and in vivo Matrigel plug assay. Together, these results indicate that ahMNCs have significant therapeutic efficacy in SCI via replacement of damaged neural cells and pro-angiogenic effects on the microenvironment of SCI.

【 授权许可】

Unknown