Long-term affected flat oyster (Ostrea edulis) haemocytes show differential gene expression profiles from naïve oysters in response to Bonamia ostreae

Genomics

A. Cao^2¹  P. Ronza^1²  M.I. Quiroga^1³  J.A. Álvarez-Dios^5⁴  A. Gómez-Tato^4⁵  A. Villalba^2,7⁶  A.F.M. Hasanuzzaman^6⁷  D. Robledo^3⁸ 

[1] Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galicia, 36620 Vilanova de Arousa, Spain^2;Departamento de Anatomía, Producción Animal e Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain^1;Departamento de Ciencias de la Vida, Universidad de Alcalá, 28871 Alcalá de Henares, Spain^7;Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidade de Santiago de Compostela, 15781 Santiago de Compostela, Spain^5;Departamento de Matemáticas, Facultad de Matemáticas, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain^4;Departamento de Zoología, Genética y Antropología Física, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain^8;Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna 9208, Bangladesh^6;The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK^3

关键词: Bonamiosis;    Parasite;    Oligo-microarray;    Immune response;    Mollusc;    Bivalve;   

DOI  :  10.1016/j.ygeno.2018.04.002

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

European flat oyster (Ostrea edulis) production has suffered a severe decline due to bonamiosis. The responsible parasite enters in oyster haemocytes, causing an acute inflammatory response frequently leading to death. We used an immune-enriched oligo-microarray to understand the haemocyte response to Bonamia ostreae by comparing expression profiles between naïve (NS) and long-term affected (AS) populations along a time series (1 d, 30 d, 90 d). AS showed a much higher response just after challenge, which might be indicative of selection for resistance. No regulated genes were detected at 30 d in both populations while a notable reactivation was observed at 90 d, suggesting parasite latency during infection. Genes related to extracellular matrix and protease inhibitors, up-regulated in AS, and those related to histones, down-regulated in NS, might play an important role along the infection. Twenty-four candidate genes related to resistance should be further validated for selection programs aimed to control bonamiosis.

【 授权许可】

CC BY