Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Biotechnology for Biofuels

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[1] 0000 0001 2173 3359, grid.261112.7, Department of Bioengineering, Northeastern University, 02115, Boston, MA, USA;0000 0001 2173 3359, grid.261112.7, Department of Chemistry and Chemical Biology, Northeastern University, 02115, Boston, MA, USA;0000 0004 1936 9676, grid.133342.4, Department of Chemistry and Biochemistry, University of California, Santa Barbara, 93106, Santa Barbara, CA, USA;0000 0004 1937 2197, grid.169077.e, Bindley Bioscience Center, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 2197, grid.169077.e, Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA;0000 0001 2173 6074, grid.40803.3f, Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Room 3227, 28081, Kannapolis, NC, USA;0000 0004 1937 2197, grid.169077.e, Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1936 8091, grid.15276.37, Department of Horticulture, University of Florida, P.O. Box 110690, 1253 Fifield Hall, 32611, Gainesville, FL, USA;0000 0004 1937 2197, grid.169077.e, Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 2197, grid.169077.e, Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 2197, grid.169077.e, Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA;Purdue Center for Plant Biology, West Lafayette, USA;0000 0004 1937 2197, grid.169077.e, Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA;Purdue Center for Plant Biology, West Lafayette, USA;0000 0004 1937 2197, grid.169077.e, Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA;0000 0001 2297 5165, grid.94365.3d, National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA;0000 0004 1937 2197, grid.169077.e, Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 0722, grid.11899.38, Department of Food Science and Experimental Nutrition, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 14, 05508-000, São Paulo, SP, Brazil;0000 0004 1937 2197, grid.169077.e, Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA;Purdue Center for Plant Biology, West Lafayette, USA;0000 0004 1937 2197, grid.169077.e, Department of Chemistry, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 2197, grid.169077.e, Department of Forestry and Natural Resources, Purdue University, 47907, West Lafayette, IN, USA;0000 0001 2151 0999, grid.411017.2, Department of Food Science, University of Arkansas, 72701, Fayetteville, AR, USA;0000 0004 1937 2197, grid.169077.e, Department of Forestry and Natural Resources, Purdue University, 47907, West Lafayette, IN, USA;Purdue Center for Plant Biology, West Lafayette, USA;0000 0004 1937 2197, grid.169077.e, Laboratory of Renewable Resource Engineering (LORRE), Department of Agricultural and Biological Engineering, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1937 2197, grid.169077.e, Laboratory of Renewable Resource Engineering (LORRE), Department of Agricultural and Biological Engineering, Purdue University, 47907, West Lafayette, IN, USA;0000 0004 1759 700X, grid.13402.34, College of Biosystems Engineering and Food Science, Zhejiang University, 38 Zheda Rd, Xihu Qu, 310027, Hangzhou Shi, Zhejiang Sheng, China;National Renewable Energy Laboratory, Biosciences Center, 80401, Golden, CO, USA;

关键词: Cellulose;    Lignin;    Recalcitrance;    Catalysis;    Delignification;    Poplar;   

DOI  :  10.1186/s13068-019-1503-y

来源: publisher

【 摘 要 】

BackgroundLow-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymethylfurfural (HMF) and levulinic acid (LA). However, lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula × P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. Previous studies have established that wood particles from transgenic lines of hybrid poplar with high syringyl (S) lignin content give greater glucose yields following enzymatic digestion.ResultsLow-temperature (− 20 °C) treatment of S-lignin-rich poplar wood particles in TFA slightly increased yields of glucose from enzymatic digestions and HMF and LA from maleic acid–AlCl3 catalysis. Subsequent gelatinization at 55 °C resulted in over 80% digestion of cellulose in only 3 to 6 h with high-S-lignin wood, compared to 20–60% digestion in the wild-type poplar hybrid and transgenic lines high in guaiacyl lignin or 5-hydroxy-G lignin. Disassembly of lignin in woody particles by Ni/C catalytic systems improved yields of glucose by enzymatic digestion or catalytic conversion to HMF and LA. Although lignin was completely removed by Ni/C-catalyzed delignification (CDL) treatment, recalcitrance to enzymatic digestion of cellulose from the high-S lines was reduced compared to other lignin variants. However, cellulose still exhibited considerable recalcitrance to complete enzymatic digestion or catalytic conversion after complete delignification. Low-temperature swelling of the CDL-treated wood particles in TFA resulted in nearly complete enzymatic hydrolysis, regardless of original lignin composition.ConclusionsGenetic modification of lignin composition can enhance the portfolio of aromatic products obtained from lignocellulosic biomass while promoting disassembly into biofuel and bioproduct substrates. CDL enhances rates of enzymatic digestion and chemical conversion, but cellulose remains intrinsically recalcitrant. Cold TFA is sufficient to overcome this recalcitrance after CDL treatment. Our results inform a ‘no carbon left behind’ strategy to convert total woody biomass into lignin, cellulose, and hemicellulose value streams for the future biorefinery.

【 授权许可】

CC BY