Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis

BMC Infectious Diseases

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[1] 0000 0000 9688 4664, grid.472872.c, Fundação Ezequiel Dias (FUNED), Belo Horizonte, MG, Brazil;0000 0001 0723 0931, grid.418068.3, Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ, Brazil;0000 0001 2181 4888, grid.8430.f, Escola de Veterinária (UFMG), Belo Horizonte, MG, Brazil;0000 0001 2181 4888, grid.8430.f, Faculdade de Farmácia (UFMG), Belo Horizonte, MG, Brazil;0000 0001 2181 4888, grid.8430.f, Laboratório de Pesquisa em Micobactérias, Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil;

关键词: Tuberculosis;    Mycobacterium tuberculosis;    Multidrug-resistant tuberculosis;    Genotype;    Single nucleotide polymorphisms;   

DOI  :  10.1186/s12879-019-4152-7

来源: publisher

【 摘 要 】

BackgroundIn recent decades, Mycobacterium tuberculosis with the RDRio genotype, frequently isolated from tuberculosis patients in Rio de Janeiro, has become part of the Latin American – Mediterranean (LAM) family and has been associated with multidrug-resistant tuberculosis (MDR-TB). The aim of this study was to investigate the frequency of M. tuberculosis RDRio in the state of Minas Gerais, Brazil, and its relationship with MDR-TB.MethodsFor convenience, 172 susceptible and 63 MDR M. tuberculosis isolates were taken from pulmonary samples from patients diagnosed between January 2007 and December 2011. The DNA extracted from these isolates was analyzed by spoligotyping, PCR-RFLP to characterize fbpC103/Ag85C103, multiplex PCR to detect RDRio and RD174, and MIRU-VNTR 24 loci.ResultsAmong the 235 isolates, the RDRio pattern was identified in 122 (51.9%) isolates (IC 0.45–0.58), with 100 (42.5%) wild-type and 13 (5.5%) mixed pattern isolates, whereas RD174 was identified in 93 of the 122 RDRio positive samples (76.3%). The LAM family and the LAM9 lineage were the most frequently identified among the isolates in this study. Among the 63 MDR isolates, 41 (65.1%) were RDRio and 28 (44.4%) RD174.ConclusionThe association of both deletions with MDR proved to be statistically significant, corroborating the few reports that have associated RDRio with MDR.

【 授权许可】

CC BY