Generation of functional dopaminergic neurons from human spermatogonial stem cells to rescue parkinsonian phenotypes

Stem Cell Research & Therapy

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[1] 0000 0001 0599 1243, grid.43169.39, Department of Foot and Ankle Surge, Hong Hui Hospital, Xi’an Jiaotong University, 710054, Xi’an, China;0000 0001 0599 1243, grid.43169.39, Department of Spine Surgery, Hong Hui Hospital, Xi’an Jiaotong University, 710054, Xi’an, China;0000 0001 0599 1243, grid.43169.39, Translational Medicine Center, Hong Hui Hospital, Xi’an Jiaotong University, 710054, Xi’an, China;0000 0001 0599 1243, grid.43169.39, Translational Medicine Center, Hong Hui Hospital, Xi’an Jiaotong University, 710054, Xi’an, China;0000 0001 0599 1243, grid.43169.39, Department of Spine Surgery, Hong Hui Hospital, Xi’an Jiaotong University, 710054, Xi’an, China;

关键词: Human spermatogonial stem cells;    Direct conversion;    Dopaminergic neurons;    Neurophysiology activity, cell transplantation;    Parkinson’s disease;   

DOI  :  10.1186/s13287-019-1294-x

来源: publisher

【 摘 要 】

BackgroundRecent progress in the induced generation of dopaminergic (DA) neurons from different types of stem cells or reprogrammed somatic cells holds tremendous potential for the treatment of Parkinson’s disease (PD). However, the lack of a reliable source for cell replacement therapy remains a major limitation in the treatment of human neurological disorders. Additionally, the current protocols for in vitro differentiation or cell reprogramming to generate human DA neurons are laborious, time-consuming, and expensive, and efficient conversion of human spermatogonial stem cells (hSSCs) to functional DA neurons has not yet been achieved.MethodsPrimary hSSCs from testicular tissues of patients were exposed to an improved induction system, which consisted mainly of olfactory ensheathing cell conditioned culture medium (OECCM) and a set of defined cell-extrinsic factors and small molecules. Morphological changes were assessed, along with the expression of various DA neuron phenotypic markers (e.g., Tuj-1, TH, Nurr1, DAT) and several critical pro-DA neurogenesis effectors (e.g., EN-1, Pitx3, Foxa2, Lmx1a, Lmx1b, and OTX2). In addition, transcriptome analysis was used to further evaluate the genetic similarity between the artificially differentiated DA neurons and genuine ones. Concomitantly, the functional properties of converted DA neurons including synapse formation, dopamine release, electrophysiological activity, and neuron-specific Ca2+ signaling images were determined. Finally, hSSCs in the early stage of induction were evaluated for survival, differentiation, migration, tumorigenicity in the mouse striatum, and improvement of functional deficits in MPTP-induced PD animals.ResultsThe hSSC-derived neurons not only acquired neuronal morphological features but also expressed various phenotypic genes and protein characteristic of DA neurons and several effectors critical for pro-DA neurogenesis. Strikingly, as the period of induction was prolonged, expression of the critical molecules for DA neuron epigenetic status gradually increased while hSSC-specific markers sharply decreased. After 3 weeks of induction, the transdifferentiation efficiency reached 21%. In addition, hierarchical clustering analysis showed that the differentiated DA neurons closely resembled genuine ones. Furthermore, the hSSC-derived neurons gained sophisticated functional properties of wild-type DA neurons, and pro-induced hSSCs efficiently survived, migrated, and differentiated into DA neurons without tumorigenesis after transplantation into mouse striatum, leading to improvement of functional deficits in PD animals.ConclusionsThe results showed that, using the present improved straightforward approach, hSSCs could acquire DA neuron morphological features and functional properties and rescue parkinsonian phenotypes. Our strategy for the conversion of hSSCs into DA neurons is very efficient and thus may provide an alternative approach suitable for clinical cell therapy to treat neurodegenerative diseases including PD.

【 授权许可】

CC BY