期刊论文详细信息
Nanoscale Research Letters
Gastric Parietal Cell and Intestinal Goblet Cell Secretion: a Novel Cell-Mediated In Vivo Metal Nanoparticle Metabolic Pathway Enhanced with Diarrhea Via Chinese Herbs
  1    2    3    3    3    3    3    3    3    3    4    4    5    5    5 
[1] 0000 0001 2171 1133, grid.4868.2, School of Engineering and Materials Science, Queen Mary University of London, London, UK;0000 0004 0368 8293, grid.16821.3c, Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, 200336, Shanghai, People’s Republic of China;0000 0004 0368 8293, grid.16821.3c, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People’s Republic of China;0000 0004 0368 8293, grid.16821.3c, Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Thin Film and Microfabrication Key Laboratory of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electronical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People’s Republic of China;0000 0004 0368 8293, grid.16821.3c, Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Thin Film and Microfabrication Key Laboratory of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electronical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People’s Republic of China;0000 0004 0368 8293, grid.16821.3c, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People’s Republic of China;0000 0004 1803 4911, grid.410740.6, State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, 100850, Beijing, People’s Republic of China;
关键词: Triangular silver nanoplates;    Magnetic nanoparticles;    Au nanoclusters;    Au nanorods;    Goblet cells;    Parietal cells intestinal excretion;    CBD ligation;   
DOI  :  10.1186/s11671-019-2908-z
来源: publisher
PDF
【 摘 要 】

Up to date, the way in which metal nanoparticles are cleared in vivo has yet to be elucidated well. Herein, we report a novel intestinal goblet cell-mediated in vivo clearance pathway to remove metal nanoparticles. Typical metal nanoparticles such as triangular silver nanoplates, magnetic nanoparticles, gold nanorods, and gold nanoclusters were selected as representative examples. These metal nanoparticles were prepared, characterized, and injected via tail vein into a mice model with common bile duct (CBD) ligation. The feces and urines were collected for 7 days to be followed by the sacrifice of the mice and collection of the intestinal and gastric tissues for further analysis. The results showed that all four selected metal nanoparticles were located inside the goblet cells (GCs) of the whole intestinal tissue and were excreted into the gut lumen through the secretion of intestinal GC. Moreover, triangular silver nanoplates and gold nanorods were located inside the gastric parietal cells (PCs). Importantly, nanoparticles did not cause obvious pathological changes in intestinal tissues. In this study, we confirmed that the blood corpuscles are involved in the GCs secretion pathway. Furthermore, we found that the secretion of nanoparticles from intestinal GCs and PCs is accelerated by diarrhea induced via Chinese herbs. In conclusion, metal nanoparticles such as triangular silver nanoplates, magnetic nanoparticles, gold nanorods, and gold nanoclusters can be cleaned away by intestinal GCs and PCs. This novel pathway of in vivo clearance of metal nanoparticles has a great potential for future applications such as new drug design and development, nanoparticle-based labeling and in vivo tracking, and biosafety evaluation of in vivo nanoparticles.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO201909243012860ZK.pdf 2822KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:11次