GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila

G3: Genes, Genomes, Genetics

article

Juan J. Pérez-Moreno¹  Cahir J. O’Kane¹ 

[1] Department of Genetics, University of Cambridge, United Kingdom, CB2 3EH

关键词: Drosophila;    GAL4;    neuromuscular junction;    NMJ;    synaptic boutons;    glutamatergic synapse;    motor neuron;   

DOI  :  10.1534/g3.118.200809

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons.

【 授权许可】

CC BY|CC BY-NC