Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59

G3: Genes, Genomes, Genetics

article

Zhen Xu¹  Raju Misra³  Dorota Jamrozy⁴  Gavin K. Paterson⁵  Ronald R. Cutler²  Mark A. Holmes⁶  Saheer Gharbia³  Hermine V. Mkrtchyan¹ 

[1] Department of Public Health, National Demonstration Center for Experimental Preventive Medicine Education, Tianjin Medical University, Tianjin, China 300070;School of Biological and Chemical Sciences, Queen Mary University of London, London, UK E1 4NS;Natural History Museum, Core Research Laboratories, Molecular Biology, London, UK SW7 5BD;The Wellcome Trust Sanger Institute, Cambridge, UK CB10 1SA;The Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, UK EH8 9LE;Department of Veterinary Medicine, University of Cambridge, Cambridge, UK CB3 0ES

关键词: Staphylococcus epidermidis;    antibiotic resistance;    whole genome sequence;    comparative analysis;   

DOI  :  10.1534/g3.118.200314

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

Staphylococcus epidermidis is a major opportunistic pathogen primarily recovered from device-associated healthcare associated infections (DA-HAIs). Although S. epidermidis and other coagulase-negative staphylococci (CoNS) are less virulent than Staphylococcus aureus , these bacteria are an important reservoir of antimicrobial resistance genes and resistance-associated mobile genetic elements that can be transferred between staphylococcal species. We report a whole genome sequence of a multidrug resistant S. epidermidis (strain G6_2) representing multilocus sequence type (ST) 59 and isolated from an environmental sampling of a hotel room in London, UK. The genome of S. epidermidis G6_2 comprises of a 2408357 bp chromosome and six plasmids, with an average G+C content of 32%. The strain displayed a multi-drug resistance phenotype which was associated with carriage of 7 antibiotic resistance genes ( blaZ , mecA , msrA , mphC , fosB , aacA-aphD , tetK ) as well as resistance-conferring mutations in fusA and ileS . Antibiotic resistance genes were located on plasmids and chromosome. Comparative genomic analysis revealed that antibiotic resistance gene composition found in G6_2 was partly preserved across the ST59 lineage.

【 授权许可】

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