Cohesin Function in Cohesion, Condensation, and DNA Repair Is Regulated by Wpl1p via a Common Mechanism in Saccharomyces cerevisiae

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

article

Michelle S. Bloom¹  Douglas Koshland¹  Vincent Guacci¹ 

[1] Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

关键词: sister chromatid cohesion;    condensation;    DNA repair;    cohesin;    Wpl1;   

DOI  :  10.1534/genetics.117.300537

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Cohesin tethers DNA to mediate sister chromatid cohesion, chromosome condensation, and DNA repair. How the cell regulates cohesin to perform these distinct functions remains to be elucidated. One cohesin regulator, Wpl1p , was characterized in Saccharomyces cerevisiae as a promoter of efficient cohesion and an inhibitor of condensation. Wpl1p is also required for resistance to DNA-damaging agents. Here, we provide evidence that Wpl1p promotes the timely repair of DNA damage induced during S-phase. Previous studies have indicated that Wpl1p destabilizes cohesin’s binding to DNA by modulating the interface between the cohesin subunits Mcd1p and Smc3p . Our results suggest that Wpl1p likely modulates this interface to regulate all of cohesin’s biological functions. Furthermore, we show that Wpl1p regulates cohesion and condensation through the formation of a functional complex with another cohesin-associated factor, Pds5p . In contrast, Wpl1p regulates DNA repair independently of its interaction with Pds5p . Together, these results suggest that Wpl1p regulates distinct biological functions of cohesin by Pds5p -dependent and -independent modulation of the Smc3p / Mcd1p interface.

【 授权许可】

CC BY