Acetylation-Dependent Recruitment of the FACT Complex and Its Role in Regulating Pol II Occupancy Genome-Wide in Saccharomyces cerevisiae

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

article

Rakesh Pathak¹  Priyanka Singh¹  Sudha Ananthakrishnan¹  Sarah Adamczyk¹  Olivia Schimmel¹  Chhabi K. Govind¹ 

[1] Department of Biological Sciences, Oakland University, Rochester, Michigan 48085

关键词: Spt6;    Spt16;    FACT;    transcription elongation;    histone chaperones;    Gcn4;    Saccharomyces cerevisiae;    Gcn5;    Esa1;    histone acetyltransferases;    genome-wide;    ChIP-chip;   

DOI  :  10.1534/genetics.118.300943

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Histone chaperones, chromatin remodelers, and histone modifying complexes play a critical role in alleviating the nucleosomal barrier for DNA-dependent processes. Here, we have examined the role of two highly conserved yeast ( Saccharomyces cerevisiae ) histone chaperones, facilitates chromatin transcription (FACT) and Spt6 , in regulating transcription. We show that the H3 tail contributes to the recruitment of FACT to coding sequences in a manner dependent on acetylation. We found that deleting a H3 histone acetyltransferase Gcn5 or mutating lysines on the H3 tail impairs FACT recruitment at ADH1 and ARG1 genes. However, deleting the H4 tail or mutating the H4 lysines failed to dampen FACT occupancy in coding regions. Additionally, we show that FACT depletion reduces RNA polymerase II (Pol II) occupancy genome-wide. Spt6 depletion leads to a reduction in Pol II occupancy toward the 3′-end, in a manner dependent on the gene length. Severe transcription and histone-eviction defects were also observed in a strain that was impaired for Spt6 recruitment ( spt6 Δ202) and depleted of FACT. Importantly, the severity of the defect strongly correlated with wild-type Pol II occupancies at these genes, indicating critical roles for Spt6 and Spt16 in promoting high-level transcription. Collectively, our results show that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription.

【 授权许可】

CC BY