Scutia buxifolia Reiss inhibit platelet aggregation and alters the activities of enzymes that hydrolyze adenine nucleotides in lymphocytes and platelets

Journal of Applied Biomedicine

关键词: Medicinal plant;    Rhamnaceae;    Ectonucleotidases;    Platelets;    Lymphocytes;    Platelet aggregation;    HPLC/DAD;   

DOI  :  10.1016/j.jab.2014.05.001

学科分类：医学（综合）

来源: Elsevier

【 摘 要 】

Platelets plays a central role in hemostatic processes and consequently are similarly involved in pathological processes, such as arterial thrombosis and atherosclerosis. In this study we investigate the effect of aqueous crude extracts of Scutia buxifolia on NTPDase and 5′-nucleotidase activity on platelets and lymphocytes as well as the profile of the platelet aggregation. The effect of the aqueous crude extract obtained from S. buxifolia leaves (SbL) and stem bark (SbS) on enzymatic activities and platelet aggregation was investigated by in vitro tests. The platelets and lymphocytes were exposed to aqueous extracts of S. buxifolia at concentrations 1–200 μg/mL in the presence of ATP, ADP, AMP as substrates. The results showed that SbS and SbL potentially inhibited the NTPDase and 5′-nucleotidase in platelets and lymphocytes. Moreover, we found that ADP-induced aggregation was only inhibited by the SbS. This data suggest the existence of compounds in S. buxifolia that may decrease the NTPDase and 5′-nucleotidase activity, causing peripheral alterations in adenine nucleotide levels and protection against ADP-induced platelet aggregation.

【 授权许可】

Unknown