RGD peptide doped polypyrrole film as a biomimetic electrode coating for impedimetric sensing of cell proliferation and cytotoxicity

Journal of Applied Biomedicine

关键词: RGD peptide;    Polypyrrole;    Cell-based sensor;    Proliferation;    Cytotoxicity;    Electrochemical impedance microscopy;   

DOI  :  10.1016/j.jab.2017.06.001

学科分类：医学（综合）

来源: Elsevier

【 摘 要 】

A novel and facile in vitro cell sensing system has been developed with one-step electropolymerization of the conducting polypyrrole(PPy) polymer using RGD peptide as the sole dopant on an indium tin oxide (ITO) surface. The resulted RGD peptide-doped polypyrrole (PPy/RGD) composite film had a robust surface, in which PPy provided a biocompatible matrix for cell growth and a conducting interface for electrical detection, while the RGD peptide entrapped in the PPy matrix conferred the desired biomimetic properties. Using the human lung cancer cell A549 as a model, this system can be used to monitor cell behaviors of proliferation and cytotoxicity.

【 授权许可】

Unknown