Isolation of Collagen from Marine Resources from the Black Sea

Current Health Sciences Journal

MELAT CHERIM ; RODICA SIRBU ; IONELA BELU

关键词: marine collagen;    collagen hydrolysates;    tissue regeneration;   

学科分类：医学（综合）

来源: Universitatea de Medicina si Farmacie din Craiova / University of Medicine and Pharmacy

【 摘 要 】

Purpose: Evaluation of possibilities of obtaining type I fibrillar collagen from fish skin using the Black Sea marine resources and suitable cross-linking agent for this one to stay stable at body or room temperature. Materials and methods: Collagen has been extracted from the skin of the Grey mullet and Sturgeon by the acidic method with Acetic acid 0.5M. Cross-linking has been performed by using Tanic acid at 4-6oC. The rheological behaviour was determined by using a Haake VT 550 rheo-viscometer. Results: The collagen was isolated from the skin of Gray mullet and Sturgeon. (Acipenser gueldenstaedtii). Hydrogels have a pseudoplastic behavior. Tanic acid was used for cross-linking like a better alternative, eliminating the toxicity of glutaraldehyde. Conclusion. The yield of collagen extraction was higher for the Gray mullet skin, than Sturgeon. Pseudo-plastic behavior allows them to be successfully applied in the treatment of wounds. The isolated type I collagen may serve as an attractive alternative to mammalian collagen for biomedical and pharmaceutical applications.

【 授权许可】

Unknown