Advanced Microspheres as Injectable Cell Carriers for Tissue Engineering.

tissue regeneration;cell carrier;stem cell;polymer self-assembly;biomimetic;drug delivery;Biomedical Engineering;Engineering;Biomedical Engineering

Zhang, ZhanpengKim, Jinsang ;

University of Michigan

关键词: tissue regeneration;    cell carrier;    stem cell;    polymer self-assembly;    biomimetic;    drug delivery;    Biomedical Engineering;    Engineering;    Biomedical Engineering;   

Others  :  https://deepblue.lib.umich.edu/bitstream/handle/2027.42/111559/zzp_1.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Biodegradable polymer microspheres have emerged as injectable cell carriersfor the regeneration and repair of irregularly-shaped tissue defects. The physical structure and chemical composition of the microsphere are critical to its function and performance. However, it is challenging to manipulate the physical structure of microspheres at various length scales and introduce desirable chemistry on the microspheres for bioconjugation at the same time. In this thesis, the author develops a series of versatile techniques, including polymer self-assembly and novel emulsification methods, to simultaneously control the physical and chemical structure of spheres. Firstly, the author investigates the self-assembly of star-shaped polymers at both the nano- and micro-meter scales, and develops a versatile method to fabricate microspheres with simultaneous control over the nano- and micro-meter scale features. Secondly, the author summarizes a more generalized emulsification technique to produce nano- and micro-structured spheres from various types of polymers. Based on the discovered principles of microsphere assembly, the author builds a functional nanofibrous hollow microsphere platform, which can conjugate biomolecules and guide stem cell dierentiation for cartilage and bone tissue enigneering. Last but not the least, the author describes the use of the unique nanofibrous spongy micro-spheres for human dental pulp stem cell delivery and dental pulp regeneration. These new microcarriers also show great potential for other applications in tissue regeneration and biomolecule deliveries.