Biofilm formation by Mycoplasma fermentans on intrauterine devices

International Journal of Medicine and Medical Sciences

Silvia Giono¹  Lilia Cedillo²  Antonio Rivera²  Nadia Rodriguez³ 

[1] Bacteriology Laboratory, Instituto Polit茅cnico Nacional, M茅xico, D.F.;Microbiological Research Center, Benem茅rita Universidad Aut贸noma de Puebla. M茅xico.;Tropical Medicine Institute Pedro Kouri, Habana-Cuba.

关键词: Adherence;    biofilm;    Mycoplasma fermentans;    intrauterine devices.;   

学科分类：医学（综合）

来源: Academic Journals

【 摘 要 】

Microbial biofilm are communities of sessile microorganisms formed by cells that are attached irreversibly to a substratum or interface or to each other and embedded in hydrated matrix of extracellular polymeric substances. Bacterial biofilm formation on intrauterine devices is a very important event in the pathogenesis and evolution of infections associated with the use of these medical devices. Mycoplasmas are typical surface parasites colonizing the mucous membranes of animals and man. Efficient adherence mechanisms are therefore a prerequisite for survival and, in some species, for pathogenicity. The purpose of this study was to examine biofilm formation by Mycoplasma fermentans on intrauterine devices. Intrauterine devices were placed in M. fermentans cultures during 72 h. Mycoplasmas were analyzed for biofilm formation and cell counts compared for both biofilm and planktonic cells. The examination was carried with stereoscopic and scanning electron microscope. Crystal violet staining and scanning electron microscopic analysis of intrauterine devices indicated that M. fermentans formed a biofilm. This study might allow for clearer understanding of the rate of biofilm formation and the importance of different materials, contaminating organisms, and treatments which could control the process.

【 授权许可】

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