A conserved distal segment of the mouse CSF‐1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic mice

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: transgenic;    knockout mice;   

DOI  :  10.1189/jlb.0809557

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Csf1rmRNAinadultmiceisexpressedincellsofthemacrophagelineage,andduringdevelopment,itisalsoexpressedfromaseparatepromoterinplacentaltrophoblastcells.Thismousetrophoblastpromotersequenceisconservedacrossspecies,buthumantrophoblastsactuallyinitiatetranscriptionfromaseparatepromoter20kbupstream,whichisnotconservedinrodents.A7.2‐kbfragmentofthemouseCsf1rgenomicDNA,includingthe3.5‐kbpromoter,thefirstcodingexonanddownstreamintron,issufficienttodirectreproducibleposition‐andcopynumber‐independentexpressionofanEGFPreporterinvitroandinvivo.Inthisstudy,wehaveexaminedtheconsequenceofremovalofthe150‐bpfragmentencompassingtheconservedtrophoblastpromoterregioninthecontextofthe7.2‐kbpromoteronreportergeneexpressionintransgenicmice.ThedeletionablatedexpressionintheplacentabutalsoabolishedexpressioninmultinucleatedOCLandreducedexpressioninmacrophages.RT‐PCRanalysesofCsf1rmRNArevealedthatmouseOCLuseanotherpromoterwithinthisregion,distinctfromthatusedinplacentaltrophoblasts,togenerateanalternative5′UTR...

【 授权许可】

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