Neutrophil extracellular trap formation is elicited in response to cold physical plasma

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: kINPen;    reactive species;    wound healing;   

DOI  :  10.1189/jlb.3A0415-165RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Coldphysicalplasmaisanionizedgaswithamultitudeofcomponents,includinghydrogenperoxideandotherreactiveoxygenandnitrogenspecies.Recentstudiessuggestthatexposureofwoundstocoldplasmamayacceleratehealing.Uponwounding,neutrophilsarethefirstlineofdefenseagainstinvadingmicroorganismsbuthavealsobeenidentifiedtoplayaroleindelayedhealing.Inthisstudy,weexaminedhowplasmatreatmentaffectsthefunctionsofperipheralbloodneutrophils.Plasmatreatmentinducedoxidativestress,asassessedbytheoxidationofintracellularfluorescentredoxprobes;reducedmetabolicactivity;butdidnotinduceearlyapoptosis.Neutrophiloxidativeburstwasonlymodestlyaffectedafterplasmatreatment,andthekillingofPseudomonasaeruginosaandStaphylococcusaureuswasnotsignificantlyaffected.Intriguingly,wefoundthatplasmainducedprofoundextracellulartrapformation.Thiswasinhibitedbythepresenceofcatalaseduringplasmatreatmentbutwasnotreplicatedbyaddinganequivalentconcentrationofhydrogenperoxide.Plasma‐inducedneutrophilextracellulartrapformationwasnotdependentontheactivityofmyeloperoxidaseorNADPHoxidase2butseemedtoinvolveshort‐livedmolecules.TheamountofDNAreleaseandthetimecourseafterplasmatreatmentweresimilartothatwiththecommonneutrophilextracellulartrapinducerPMA.Afterneutrophilextracellulartrapshadformed,concentrationsofIL‐8werealsosignificantlyincreasedinsupernatantsofplasma‐treatedneutrophils.BothneutrophilextracellulartrapsandIL‐8releasemayaidantimicrobialactivityandspurinflammationatthewoundsite.Whetherthisaidsorexacerbateswoundhealingneedstobetested...

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