期刊论文详细信息
Applied Sciences
Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells
Sander Bekeschus1  Juliane Moritz1  Vandana Miller1  Rajesh Gandhirajan1  Klaus-Dieter Weltmann1  Katrin Rödder1  Hans-Robert Metelmann2 
[1] Centre for Innovation Competence (ZIK) plasmatis, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix-Hausdorff-Str.2, 17489 Greifswald, Germany;Department of Oral and Maxillofacial Surgery/Plastic Surgery, Greifswald University Medical Center, Ferdinand-Sauerbruch-Str. DZ 7, 17475 Greifswald, Germany;
关键词: kINPen;    lymphocytes;    macrophages;    plasma medicine;    reactive species;   
DOI  :  10.3390/app9040660
来源: DOAJ
【 摘 要 】

Recent advances in melanoma therapy increased median survival in patients. However, death rates are still high, motivating the need of novel avenues in melanoma treatment. Cold physical plasma expels a cocktail of reactive species that have been suggested for cancer treatment. High species concentrations can be used to exploit apoptotic redox signaling pathways in tumor cells. Moreover, an immune-stimulatory role of plasma treatment, as well as plasma-killed tumor cells, was recently proposed, but studies using primary immune cells are scarce. To this end, we investigated the role of plasma-treated murine B16F10 melanoma cells in modulating murine immune cells’ activation and marker profile. Melanoma cells exposed to plasma showed reduced metabolic and migratory activity, and an increased release of danger signals (ATP, CXCL1). This led to an altered cytokine profile with interleukin-1β (IL-1β) and CCL4 being significantly increased in plasma-treated mono- and co-cultures with immune cells. In T cells, plasma-treated melanoma cells induced extracellular signal-regulated Kinase (ERK) phosphorylation and increased CD28 expression, suggesting their activation. In monocytes, CD115 expression was elevated as a marker for activation. In summary, here we provide proof of concept that plasma-killed tumor cells are recognized immunologically, and that plasma exerts stimulating effects on immune cells alone.

【 授权许可】

Unknown   

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