Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: M‐;    CSF;    GM‐;    CSF;    IL‐;    4;    MHC II;    dendritic cells;    aortic ring;    collagen;   

DOI  :  10.1189/jlb.0310178

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Angiogenesisintheaorticringmodelisprecededbyactivationoftheimmunesystemandimpairedbyablationofadventitialmacrophages.TreatmentofaorticcultureswithM‐CSFinducedextensiveperiaorticoutgrowthofCD45+CD68+mononuclearcellswithultrastructuralfeaturesofmacrophagesandDCs.PeriaorticlysisofcollagencausedmanyCD45+CD68+cellstoattachtothebottomoftheculturedish.LiftingthecollagengelsleftbehindpatchesofCD45+CD68+cells,whichfocallyorganizedintobranchingcords.ThesecellsalsoexpressedCD14,CD169,F4/80,andα‐SMAbutnotCD31,vWF,desmin,orCD163.DNAsynthesisstudiesshowedthatM‐CSF‐stimulatedcellswereactivelyproliferating.AorticpatchcellsshowedphagocyticpropertiesandrespondedtoIL‐4andGM‐CSFbyexpressingMHCII,differentiatingintoDCs,andformingmultinucleatedgiantcells.TheyalsostimulatedangiogenesisandVEGFproductioninaorticringcultures.Thisstudydemonstratesthattherataortacontainsadistinctsubsetofimmatureimmunocytescapableofproliferating,differentiatingintomacrophagesandDCs,andstimulatingangiogenesis.IsolationofthesecellsinpatchesfromM‐CSF‐stimulatedaorticringsprovidesareproduciblesystemtostudythebiologyandangiogenicroleoftheresidentimmunesystemoftheaorticwall...

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