G‐CSF preferentially supports the generation of gut‐homing Gr‐1high macrophages in M‐CSF‐treated bone marrow cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: GM‐;    CSF;    myeloid cells;    differentiation;   

DOI  :  10.1189/jlb.1A0314-172R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TheG‐CSFisbestknownforitsactivityinthegenerationandactivationofneutrophils.Inaddition,studiesonG‐CSF−/−orG‐CSFR−/−miceandBMCculturessuggestedaroleofG‐CSFinmacrophagegeneration.However,ourunderstandingontheroleofG‐CSFinmacrophagedevelopmentislimited.Here,usinginvitroBMCmodels,wedemonstratedthatG‐CSFpromotedthegenerationofGr‐1high/F4/80+macrophage‐likecellsinM‐BMCs,likelythroughsuppressingcelldeathandenhancinggenerationofGr‐1high/F4/80+macrophage‐likecells.TheseGr‐1highmacrophage‐likecellsproduced“M2‐like”cytokinesandsurfacemarkersinresponsetoLPSandIL‐4/IL‐13,respectively.AdoptivetransferofEGFP‐expressing(EGFP+)M‐BMCsshowedadominant,gut‐homingphenotype.ThesmallintestinallaminapropriaofG‐CSFR−/−micealsoharboredsignificantlyreducednumbersofGr‐1high/F4/80+macrophagescomparedwiththoseofWTmice,butlevelsofGr‐1+/F4/80−neutrophil‐likecellsweresimilarbetweenthesemice.Collectively,theseresultssuggestanovelfunctionofG‐CSFinthegenerationofgut‐homing,M2‐likemacrophages...

【 授权许可】

CC BY