Involvement of the CX3CL1 (fractalkine)/CX3CR1 pathway in the pathogenesis of acute graft‐versus‐host disease

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: allogeneic transplant;    stem cell;    alloreactivity;    endothelium;   

DOI  :  10.1189/jlb.5HI0714-325R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThisstudyinvestigatedtheroleofcytokinesandchemokinesinaGVHDincidenceandseverityin109patientswhounderwentreduced‐intensityconditioningallogeneicstemcelltransplantation(HSCT).Amongthe42cytokinestestedatd0HSCT,onlyCX3CL1levelsatd0HSCTweresignificantlyassociatedwithGradesII–IVaGVHDdevelopment(P=0.04).IncreasedlevelsofCX3CL1atd20–30and50post‐HSCTwerealsosignificantlyassociatedwithaGVHD(P=0.02andP=0.03,respectively).Nosuchassociationwasfoundbeforetheconditioningregimenoratd100–120post‐HSCT.AsthereceptorforCX3CL1isCX3CR1,thenumberofCX3CR1+cellswasdeterminedbyflowcytometry.TheCX3CR1+CD8+TcellproportionwassignificantlyhigherinpatientswithaGVHDthanthosewithoutaGVHD(P=0.01).ToinvestigatethedistributionoftheCX3CL1/CX3CR1axisintheanatomicsitesofaGVHD,CX3CL1andCX3CR1levelswerestudiedbyuseofaninsituimmunohistochemicalanalysisonGIbiopsiesofpatientswithintestinalaGVHD.CX3CL1expressionwasincreasedsignificantlyintheepithelialcellsandmononuclearcellsofthelaminapropria.CX3CR1+mononuclearcellswereidentifiedinclosecontactwithepithelialcells.ThesefindingsstronglysuggesttheimplicationoftheCX3CL1/CX3CR1axisinthepathogenesisofaGVHD...

【 授权许可】

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