Activation‐induced accumulation of B and T lymphocyte attenuator at the immunological synapse in CD4+ T cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: T cells;    costimulation;    cell‐;    surface molecules;    trafficking;   

DOI  :  10.1189/jlb.0309138

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

BTLA,arecentlyclonedcoreceptorexpressedonlymphocytes,negativelyregulatescellactivationbyrecruitingSHP‐1/SHP‐2.However,themechanismsthatregulatetheintracellularlocalizationofBTLAanditstraffickingtothecellsurfaceinTcellsarestillunknown.TodeterminethemechanismsthatregulatetheexpressionofBTLAonthesurfaceofTcells,weexaminedthesubcellularlocalizationofBTLAinmouseTcellsinasteadystate,aswellasuponactivationbyusingaconfocallaser‐scanningmicroscopy.WefoundthatBTLAwaslocalizedmainlyintheGolgiapparatusandsecretorylysosomesinrestingCD4+Tcells.WealsofoundthatintracellularBTLAwastranslocatedtothecellsurfaceandaccumulatedattheimmunologicalsynapseuponTCRstimulation.Furthermore,wefoundthattheBTLA‐HVEMinteractionwasrequiredfortheassociationofBTLAwithlipidrafts.TheseresultsindicatethatthesurfaceexpressionofBTLAanditsaccumulationattheimmunologicalsynapsearetightlyregulatedbyTCRandHVEMstimulationtodeliverefficientinhibitorysignalsintheregulationofCD4+Tcellactivation...

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