CD4 T cell‐intrinsic IL‐2 signaling differentially affects Th1 and Th17 development

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: clonal expansion;    cytokine;    helper T cell;    IL‐;    17;    IFN‐;    γ;   

DOI  :  10.1189/jlb.1112581

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

IL‐2signalingisinvolvedinclonalexpansionofantigen‐specificCD4Tcells.IL‐2isalsoreportedtopromoteTh1butinhibitTh17differentiation,althoughinvivorelevanceremainsunclear.Inaddition,IL‐2‐dependentFoxp3+CD4TregssuppressTcellproliferation,complicatingtheinvivoroleofIL‐2inthedevelopmentofThcellresponses.Toelucidatetherolesofcell‐intrinsicIL‐2signalinginCD4Tcells,wecotransferredTCR‐TgCD4TcellsfromIL‐2Rα(CD25)‐deficientandWTmiceandanalyzeddevelopmentofantigen‐specificTh1andTh17responses.ItwasrevealedthatTh17developmentofantigen‐specificCD4Tcellswaslargelyunaffected,whereasTh1developmentwasimpairedbythelackofIL‐2signaling.SimilardatawereobtainedfrommixedBMchimeraexperimentsusingBMcellsfromCD25‐deficientandWTmice.Inaddition,althoughinvitroblockadeofIL‐2duringTh17developmentgreatlyincreasedthepercentagesofTh17cells,itdidnotaffecttheirnumbers,indicatingthatinvitroTh17developmentisalsoIL‐2‐independent.Th1developmentwasdependentonIL‐2invitroaswell.Thus,ourdatasuggestthatcell‐intrinsicIL‐2signalingiscriticalforTh1developmentbutplaysalimitedroleinTh17developmentinvitroaswellasinvivo...

【 授权许可】

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