Enhanced Toll‐like receptor (TLR) responses of TNFR‐associated factor 3 (TRAF3)‐deficient B lymphocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: lymphocyte activation;    innate immunity;    signal transduction;   

DOI  :  10.1189/jlb.0111044

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThekeyroleofTRAF6inTLRsignalingpathwaysiswellknown.MorerecentevidencehasimplicatedTRAF3asanotherTRAFfamilymemberimportanttocertainTLRresponsesofmyeloidcells.PreviousstudiesdemonstratethatTRAF3functionsarehighlycontext‐dependent,displayingreceptorandcell‐typespecificity.WethusexaminedtheTLRresponsesofTRAF3−/−mouseBlymphocytestotestthehypothesisthatTRAF3playsdistinctrolesinsuchresponses,dependingoncelltype.TRAF3−/−DCareknowntohaveadefectintype1IFNproductionandhere,showeddiminishedproductionofTNFandIL‐10andunalteredIL‐6.Inmarkedcontrast,TRAF3−/−BcellsmadeelevatedamountsofTNFandIL‐6protein,aswellasIL‐10andIP‐10mRNA,inresponsetoTLRligands.Also,incontrasttoTRAF3−/−DC,thetype1IFNpathwaywaselevatedinTRAF3−/−Bcells.IncreasedearlyresponsesofTRAF3−/−BcellstoTLRsignalswereindependentofcellsurvivalorproliferationbutassociatedwithelevatedcanonicalNF‐κBactivation.Additionally,TRAF3−/−BcellsdisplayedenhancedTLR‐mediatedexpressionofAIDandIgisotypeswitching.Thus,TRAF3playsvariedandcelltype‐specific,biologicalrolesinTLRresponses...

【 授权许可】

CC BY