Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
Dynamics of dendritic cell‐derived vesicles: high‐resolution flow cytometric analysis of extracellular vesicle quantity and quality | |
关键词: exosomes; microvesicles; immune regulation; single vesicle analysis; cognate interaction; MHC class II; | |
DOI : 10.1189/jlb.0911480 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Nano‐sizedmembranevesiclesaresecretedbymanycelltypes.Thesevesiclescanserveascarriersofcellularinformation.DC‐derivedvesiclescanbetargetedtootherimmunecellsandmodifytheirfunction.AccurateanalysisofquantitativeandqualitativechangesinEVproductionbyDCupondifferentactivationstimuliisneededtofurtherrevealtheimmuneregulatorypropertiesofDC‐derivedEVs.However,methodsforreliablequantificationofindividualEVsandforanalysisoftheheterogeneityofEVpopulationsarelimited.Withourrecentlydevelopedhigh‐resolutionflowcytometry‐basedmethod,wecanperformahigh‐throughput,multiparameter,andquantitativeanalysisofindividualEVs.Withtheuseofthisnoveltechnique,weshowthatdespitepreviousassumptions,stimulationwithbacterialLPSincreasesEVreleasebyDC.Furthermore,wedemonstrateheterogeneityinDC‐derivedEVsregardingtheirbuoyantdensityandMHCclassIIcontent.Finally,weshowthatcognateinteractionbetweenLPS‐stimulatedDCandCD4+TcellsaffectsboththequantityandqualityofLPSDC‐derivedEVspresentintheculturesupernatant.Thesedataindicatethatflowcytometry‐basedanalysisofindividualEVsisavaluable,noveltooltostudythedynamicsofEVsecretionandcomposition,offeringgreatopportunitiestounveilthefunctionofimmunecell‐derivedEVs...
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201904043460252ZK.pdf | 588KB | download |