Indoor insect allergens are potent inducers of experimental eosinophilic esophagitis in mice

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: cockroach;    dust mite;    eotaxin;    eosinophils;    mast cells;   

DOI  :  10.1189/jlb.0110025

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

EEisanemergingdiseasereportedinchildrenandadultsofurbanizedcountries,whereindoorinsectallergensaremajorhealthriskfactors.ReviewofourhospitalpatientdatabaseuncoveredthatanumberofEEpatientshavehypersensitivitytoindoorcat,dog,cockroach,anddustmiteallergens.WetestedthehypothesiswhetherinhaledindoorinsectallergensareeffectiveinducersofexperimentalEE.Wedeliveredcat,dog,cockroach,anddustmiteallergenextractsintranasallytowild‐typeandeotaxin‐1/2‐,CCR3‐,andIL‐5‐deficientmice.Interestingly,wild‐typemiceexposedtocockroachordustmiteallergensdevelopasignificantincreaseinthelevelsofesophagealeosinophilsandmastcellscomparedwithsaline‐challengedmice.Theeosinophilnumbersintheesophagusofcockroach‐anddustmite‐exposedmicewere18.3±6.8/mm2and33.4±11.1/mm2comparedwith2.3±1.8/mm2and2.1±1.2/mm2insaline‐challengedmice.Additionally,weobservedanadditiveeffectofthesetwoallergensininducingesophagealeosinophiliaandmastocytosis.Histopathologicalanalysisdetectedintraepithelialesophagealeosinophiliainmiceexposedtobothallergens.Furthermore,miceexposedtocockroachand/ordustmitehadincreasedlevelsoftotalIgEandantigen‐specificIgG1inthebloodandincreasedesophagealexpressionofeosinophil‐activecytokines(IL‐13)andchemokines(eotaxin‐1).Notably,micedeficientineotaxin‐1/2,CCR3,andIL‐5showedablatedesophagealeosinophiliafollowingcockroachordustmiteallergenexposure.ThesedataindicatethatindoorinsectallergensarepotentinducersofIL‐5andeotaxin‐mediatedesophagealeosinophilia.Theseexperimentalstudiesareinaccordancewithclinicaldatabutmayhavesomelimitationsinherenttoanimalmodelsofhumandisease...

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