Pivotal Advance: Heme oxygenase 1 expression by human CD4+ T cells is not sufficient for their development of immunoregulatory capacity

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: tolerance;    regulatory T cells;    immune modulation;    CD4+CD25+ Tregs ;    proliferation;   

DOI  :  10.1189/jlb.0508280

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

HO‐1istheonlyinducibleoneofthreeisoenzymesthatcatalyzestheoxidativedegradationofheme.HO‐1isinduciblebyvariouscellularstressfactorsandexertscytoprotectiveandimmunomodulatoryeffects.RecentpublicationsdemonstratedthatHO‐1isconstitutivelyexpressedbyCD4+CD25+TregsandinducedinCD4+CD25−TcellsuponFoxP3transfection.Here,weinvestigatedwhetherHO‐1wasessentialandsufficientforhumanTregstoexertimmunosuppressioninvitro.PGJ2inducedpronouncedexpressionofHO‐1inCD4+CD25−TcellswithoutaccompanyingFoxP3induction.TreatmentofCD4+CD25−TcellswithPGJ2decreasedtheirproliferation,whereastheHO‐1inhibitorSnPPenhancedtheproliferationofHO‐1‐expressingTregs,suggestingthatHO‐1maymodulatetheproliferativecapacityofTlymphocytes.HO‐1modulationbySnPPtreatmentofTregsorPGJ2treatmentofCD4+CD25−Tcellsneithersuppressednorinducedimmune‐modulatoryfunctioninthesecells,respectively,asmeasuredbyresponder‐cellproliferationand/orIL‐2production.Insummary,thesedatasuggestthatHO‐1expressionbyTregsmightcontributetotheirtypicalreluctancetoproliferatebutdoesnotaccountindependentlyfortheirsuppressivefunctions...

【 授权许可】

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