Functionally and morphologically distinct populations of extracellular vesicles produced by human neutrophilic granulocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: apoptotic bodies;    microvesicles;    bacterial survival;    spontaneous death;    protein profile;   

DOI  :  10.1189/jlb.3VMA1014-514R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

EVsinthemicrovesiclesizerangereleasedduringspontaneousdeathofhumanneutrophilswerecharacterizedandtheirpropertiescomparedwithpreviouslydescribedEVswithantibacterialeffect(aEVs,generatedonspecificactivation)orproducedspontaneously(sEVs).The3vesiclepopulationsoverlappedinsizeandinpartoftheconstituentproteinswerestainedwithannexinVandwereimpermeabletoPI.However,noneofthemproducedsuperoxide.Incontrast,remarkabledifferenceswereobservedinthemorphology,abundanceofproteins,andantibacterialfunction.EVsformedspontaneouslyin30min(sEVs)weremoresimilartoEVsreleasedduringspontaneousdeathin1–3dthantoEVsformedin30minonstimulationofopsoninreceptors(aEVs).SpontaneouslygeneratedEVshadnoantibacterialeffectdespitetheirlargenumberandproteincontent.Wehypothesized2parallelmechanisms:onethatproceedsspontaneouslyandproducesEVswithoutantibacterialeffectandanotherprocessthatistriggeredbyopsoninreceptorsandresultsindifferentialsortingofproteinsintoEVswithantibacterialcapacity.Ourresultscallattentiontothefunctionalandmorphologicheterogeneitywithinthemicrovesicle/ectosomefractionofEVs...

【 授权许可】

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