IL‐15 ex vivo overcomes CD4+ T cell deficiency for the induction of human antigen‐specific CD8+ T cell responses

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: primary cells;    dendritic cells;    lymphoid cell‐;    mediated immunity;   

DOI  :  10.1189/jlb.1010579

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

CD4+Thcellsareimportantfortheinductionandmaintenanceofantigen‐specificCD8+Tcellfunction,sotheirlossordysfunctioninHIV‐infectedorcancerpatientscouldreducethepatientsˈabilitytocontrolviralinfection.PreviousworkinmurinesystemsindicatedthatIL‐15codeliveredwithvaccinescouldovercomeCD4+ThcelldeficiencyforinductionoffunctionallyefficientCD8+Tcellsandmaintenanceofviral‐specificCTLs,butitsefficacyinhelpingprimaryhumanCD8+Tcellresponsesisunknown.Inthepresentstudy,apeptide‐pulsed,DC‐basedhumancocultureexvivosystemwasusedtostudytheroleofIL‐15inovercomingCD4+ThdeficiencytoelicitCD8+TcellresponsesinCD4‐depletedPBMCsfromhealthyindividualsandPBMCsfromHIV‐1‐infectedpatients.WefoundthatIL‐15couldovercomeCD4+ThdeficiencytoinduceprimaryandrecallmemoryCD8+Tcellresponsesinhealthyindividuals.Moreover,inCD4‐deficient,HIV‐1‐infectedpatientswithdiminishedCD8+Tcellresponses,IL‐15greatlyenhancedCD8+Tcellresponsestoalloantigen.TheseresultssuggestthatIL‐15maybeusefulinthedevelopmentoftherapeuticandpreventivevaccinesagainstcancersandviralinfectionsinpatientsdefectiveinCD4+Thcell...

【 授权许可】

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