Altered dynamics and differential infection profiles of lymphoid and myeloid cell subsets during acute and chronic HIV‐1 infection

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: CD16;    innate immunity;    plasmacytoid dendritic cells;   

DOI  :  10.1189/jlb.0410231

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThedynamicsofimmunecellpopulationsduringacuteHIV‐1infectionarenotfullydeciphered,especiallyfornon‐Tcells.Inthisstudy,wetestedwhetherspecificcellularsubsetsoftheinnatearmoftheimmuneresponseareaffectedearlyafterHIV‐1infection.UsingacohortofHIV‐1‐infectedindividuals,wehavemonitoredtherelativefrequencyofbloodTlymphocytes,monocytes,andDCsatvariousinfectionstagesandmeasuredtheirrespectiveintracellularHIV‐1DNAloads.TheHIV‐1DNAloadinnaiveCD4+Tlymphocytes,whicharelostveryearlyduringacuteinfection,wasten‐to100‐foldlowerthaninCD57–andCD57+memoryCD4+Tlymphocytes.Weobservedthatdespiterapid,persistentlossafterHIV‐1infection,pDCsrepresentedanon‐negligibleHIV‐1DNAreservoir.CD16+proinflammatorycDCsandmonocytesaccumulatedgradually,andHIV‐infectedCD16+monocytescontainedhigherHIV‐1DNAloadsthantheirCD16–counterpartduringacuteinfection.Duringchronicinfection,CD16+cDCsexhibitedhigherHIV‐1DNAloadsthantheCD16–population.Overall,ourresultsdemonstratethatnon‐TcellcompartmentsareamajorHIV‐1DNAreservoir,andCD16+monocytesandCD16+cDCspotentiallyplayanimportantroleinHIV‐1dissemination...

【 授权许可】

CC BY