Lipid raft‐associated β‐adducin is required for PSGL‐1‐mediated neutrophil rolling on P‐selectin

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: Src kinase;    phosphorylation;    LC‐;    MS/MS;    polarization;   

DOI  :  10.1189/jlb.2A0114-016R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Lipidrafts,aliquid‐orderedplasmamembranemicrodomain,arerelatedtocell‐surfacereceptorfunction.PSGL‐1,amajorsurfacereceptorproteinforleukocyte,alsoactsasasignalingreceptorinleukocyterolling.ToinvestigatetheroleoflipidraftinPSGL‐1signalinginhumanneutrophils,wequantitativelyanalyzedlipidraftproteomeofhumanpromyelocyticleukemiacelllineHL‐60cellsandidentifiedalipidraft‐associatedproteinβ‐adducin.PSGL‐1ligationinduceddissociationoftheraft‐associatedproteinβ‐adducinfromlipidraftsandactin,aswellasphosphorylationofβ‐adducin,indicatingatransientuncouplingoflipidraftsfromtheactincytoskeleton.Knockdownofβ‐adducingreatlyattenuatedHL‐60cellsrollingonP‐selectin.WealsoshowedthatSrckinaseiscrucialforPSGL‐1ligation‐inducedβ‐adducinphosphorylationandrelocation.Takentogether,theseresultsshowthatβ‐adducinisapivotallipidraft‐associatedproteininPSGL‐1‐mediatedneutrophilrollingonP‐selectin...

【 授权许可】

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