Transcriptomic Analysis of Mild Hypothermia-dependent Alterations During Endothelial Reperfusion Injury

Cellular Physiology and Biochemistry

关键词: Mild hypothermia;    Ischemia-Reperfusion;    Endothelial cells;    Microarray;   

DOI  :  10.1159/000315079

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background Mild hypothermia (32-34°C) improves resistance to ischemia-reperfusion (I/R) injury. However, the mechanisms by which it affects human cellular function are not fully elucidated. To further test for hypothermic modulation of global biological processes, we used DNA microarray technique to detect the overall gene expression profile. Methods Human umbilical endothelial cells (HUVECs) were incubated under control condition (37°C) or mild hypothermia (33°C) for 2 hours after stimulated ischemia. Detection of differentially expressed genes was performed with Affymetrix U133 plus 2.0 arrays and PARTEK software. We used DAVID and KEGG Pathways database to identify global trends in gene expression data. Results Our analysis has identified numerous interesting genes and processes that are differentially presented in hypothermic group when compared with normothermic control. The cell cycle was the most prominent process; several genes involved in cell apoptosis and proliferation displayed significantly differential expression; lower transcriptional level was observed for genes involved in chemokine and cell adhesion processes; genes associated with activity of transmembrane transporter and lipase were also under-expressed. Conclusion Our data indicated that mild hypothermia altered endothelial expression pattern under the condition of I/R, preferably through varying the expression of genes associated with cell cycle, apoptosis, proliferation, and inflammatory response.

【 授权许可】

CC BY-NC-ND