Tips on ligand immobilization and kinetic study using surface plasmon resonance

BioImpacts

Ezzati Nazhad Dolatabadi J¹ 

关键词: Surface plasmon resonance;    Analyte;    Ligand immobilization;    Kinetic study;   

DOI  :  10.15171/bi.2016.17

学科分类：农业科学（综合）

来源: Tabriz University of Medical Sciences and Health Services

【 摘 要 】

Surface plasmon resonance (SPR) technique offers a robust label-free approach applicable invariousinvestigations including binding affinity, specificity and kinetics of biological macromolecules (e.g., peptides, proteins and nucleotidase) and small molecules. SPR provides extremely important data on the kinetics and affinity of substances examined, through which bio-specific interaction(s) can be established by the analysis of adsorption of analyte onto the immobilized ligand(s) on a sensor-based analytical system. Due to SPR wide applications in biomedical laboratories, the aim of this editorial is to highlight the importance of SPR in affinity kinetics and ligand immobilization.

【 授权许可】

CC BY